As technologies improve the detection of specific disease biomarkers, earlier adoption of more effective targeted treatments is possible. The significance of liquid biopsies is their ability to detect early disease with just a simple blood draw. The researchers found 2,708 genes were shown to be differentially expressed when compared to samples stored in RNAlater™ and those that were flash frozen, with some of this bias attributed to the GC content of the genes, stating As a result, some researchers turn to commercially available reagents such as RNAlaterTM to stabilize nucleic acid at ambient temperature.Ī recent study from researchers at the University of Minnesota shows that storing a biological sample in RNAlaterTM could have a significant impact on the gene expression profiles when compared to samples flash frozen in liquid nitrogen. While effective, flash freezing can be overly cumbersome and impractical for outside of a controlled lab environment. One common method for stabilizing nucleic acid in biological samples is to flash-freeze the samples in liquid nitrogen.
The instability of DNA and RNA at ambient temperatures means researchers must be especially careful when collecting samples in order to ensure no loss of sample information prior to nucleic acid extraction. As one of the most powerful and prevalent tools for developing gene expression profiles, RNA-sequencing (RNA-Seq) is vulnerable to biases.
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